Serial dilution Definition. Serial dilution, as the name suggests, is a series of sequential dilutions that are performed to convert a dense solution into a more usable concentration. In simple words, serial dilution is the process of stepwise dilution of a solution with an associated dilution factor. Using Excel, plot a scatter plot of concentration vs. Absorbance using the date from the parallel and serial dilutions on the same graph. Add a best fit line and get the y = mx + b equation for each and the R value. Do the data points from both dilution series that were prepared seem to fit a straight line? Serial Vs Parallel Dilution Method Of Bod Window Awnings Sound Atomic Packing Factor For Bcc And Fcc All Mobile Price In Pakistan 2018 Blog Gilisoft Universal Keygen. Serial and parallel dilution a serial dilution is a. Map for arma 2 dayz overpoch download google. Will the dilutions be prepared in series or parallel? Serial dilution is one of the core foundational practices of homeopathy, with 'succussion', or shaking, occurring between each dilution. Therefore, this series has a constant dilution factor of.

BIOL 1406

PreLab 2.5

When do I use the serial dilution technique instead of the parallel dilution technique?

Another way to make dilutions is to use some of your existing stock solution to make a dilute solution, then use some of the dilute solution to make an even more dilute solution, then use some of that solution to make an even more dilute solution, and so on. This procedure is called the serial dilution technique.

There are two situations where serial dilutions should be used rather than parallel dilutions: Smith and wesson 9mm 3913 user manual.

FIRST: Use a serial dilution when you need several solutions of the same solute and there is a constant dilution factor. For example, suppose you have a 2 M stock solution of KMnO₄ and you want to make 15 mL of each of the following concentrations of KMnO₄: 0.2 M, 20 mM, 2 mM, and 0.2 mM. Notice that the concentration of each solution is 1/10^th the concentration of the previous solution in the series. The factor by which each solution is diluted compared to the previous one is called the dilution factor.

To calculate the dilution factor for each dilution, divide the concentration of the starting solution by the concentration of the diluted solution. For example, for the first dilution 2 M divided by 0.2 M equals 10. For the second dilution, 0.2 M divided by 20 mM equals 10. For the third dilution, 20 mM divided by
2 mM equals 10. And for the fourth dilution 2 mM divided by 0.2 mM equals 10. Therefore, this series has a constant dilution factor of 10.

SECOND

:Also use a serial dilution when the dilution factor is so large that the amount of stock solution needed to make the dilution in one step (using the formula C₁V₁ = C₂ Serial

V₂

Serial Dilution Vs Parallel Dilution

) is too small to measure accurately. Remember that the smallest volume you can measure with the micropipettors is 2 μL.

YOUR TURN
You have a stock solution of 1.6 M sucrose and you need to prepare solutions with the following concentrations of sucrose: 0.4 M, 0.1 M, 25mM, and 6.25 mM.What is the dilution factor for this series?	Hint	Check your answer.
You plan to prepare 4 solutions by serial dilution. The concentration of your stock solution is 2.7 M and the dilution factor is 3. What will be the molarity of your four solutions?	Hint	Check your answer.
You have a 1.0 M stock solution of glycine (an amino acid), and you need 5 mL of a 0.1 mM solution. By what factor do you need to dilute your stock solution?	Hint	Check your answer.
Using the formula C₁V₁ = C₂V₂, calculate the amount of stock solution you need to make 5 mL of a 0.1 mM solution from a 1.0 M stock solution. Express your answer in mL:	Hint	Check your answer.
Do you have a measuring device that will accurately measure this amount? YES NO	Hint	Check your answer.
Explain.	Hint	Check your answer.

YOUR TURN
For each of the following situations, state whether the parallel or serial dilution technique would be more appropriate, and explain your reasoning.
You need 20 mL of a 5 mM glycerol solution. You have a 1.0 M glycerol stock solution on hand. Which dilution technique is appropriate and why?	Hint	Check your answer.
You have a 2.5 M stock solution of EDTA. You need 50 mL each of 2 M, 0.5 M, 0.2 M and 0.1 M solutions of EDTA. Which dilution technique is appropriate and why?	Hint	Check your answer.
You have a 2.0 M stock solution of sucrose. You need 100 mL each of 1 M, 0.5 M, 0.25 M, 0.125 M, and 62.5 mM sucrose solutions. Which dilution technique is appropriate and why?	Hint	Check your answer.

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This section is not a recipe for your experiment. It explains someprinciples for designing dilutions that give optimal results. Onceyou understand these principles, you will be better able to designthe dilutions you need for each specific case.

Serial Dilution Problems

Often in experimental work, you need to cover a range ofconcentrations, so you need to make a bunch of differentdilutions. For example, you need to do such dilutions of thestandard IgG to make the standard curve in ELISA, and then againfor the unknown samples in ELISA.

You might think it would be good to dilute 1/2, 1/3, 1/10, 1/100.These seem like nice numbers. There are two problems with this series ofdilutions.

The dilutions are unnecessarily complicated to make. You need to do a differentcalculation, and measure different volumes, for each one. It takes a longtime, and it is too easy to make a mistake.
The dilutions cover the range from 1/2 to 1/100 unevenly.In fact, the 1/2 vs. 1/3 dilutions differ by only 1.5-fold in concentration,while the 1/10 vs. 1/100 dilutions differ by t